一氧化氮合成酶抗體說明書

更新時間：2012-06-13 瀏覽次數(shù)：1773

goat Anti-NOS

Catalog Number :HZ99762 Quantity size : 0.1ml （dilute with pH 7.4

0.01 M PBS or antibody diluent ） Background: Nitric oxide （NO） has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases （NOSs）, the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates （L-arginine, NADPH and O2） and five cofactors or prosthetic groups （FAD, FMN, calmodulin, tetrahydrobiopterin and heme）. Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including （also designated ncNOS） whose activity was first identified in neurons, and NOS3 （also designated ecNOS）, first identified in endothelial cells. The inducible form of NOS, NOS2 （also designated iNOS）, is Ca2+-independent and is expressed in a broad range of cell types..

pecificity :

goat polyclonal IgG, 200 µg/ml

recommended for detection of NOS of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine

Protein G affinity chromatography purification, purity :>95%

Isotype: IgG

Application :

Western blotting 1:100-500

Immunohistochemistry 1:100-500

ELISA 1:500-1000

Optimal working dilutions must be determined by the end user. Storage: Store at -20℃for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

人一氧化氮合成酶（NOS）elisa試劑盒 48次 1200元，96次2200元

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