Rabbit Anti-IgG英文版說明書
Catalog Number :HZ289 Quantity size : 0.1ml (dilute with pH 7.40.01 M PBS or antibody diluent )
Background: IgG is a monomeric immunoglobulin. It is the most abundantimmunoglobulin and is found in the blood and extracellular fluid. There are foursubclasses of IgG: IgG1, IgG2, IgG3 and IgG4. IgG is composed of two heavy chains(γ chains) and two light (κ or λ) chains. The chains form two domains, the Fab(antigenbinding) fragment and the Fc (constant) fragment. Each IgG Fab fragmenthas two antigen binding sites. IgG molecules are involved in secondary immuneresponse. They bind to several different kinds of pathogens, such as viruses, bacteriaand fungi, and protect the body by complement activation (the classic pathway),
opsonization for phagocytosis and neutralization of toxins. In addition, IgG is the only
isotype that can pass through the placenta, thereby providing protection to the fetus in
the first weeks of life, before the immune system of the fetus has developed.pecificity :
– Anti-IgG is a Rabbit polyclonal antibody unconjugated.
– specific for IgG of Human,Rat,pig,canine
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 25kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Thelyophilized antibody is stable at room temperature for at least one month and forgreater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01MPBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃Important Note: This product as supplied is intended for research use only, not foruse in human, therapeutic or diagnostic applications.