甘草水提物中miRNA對人免疫細(xì)胞基因表達(dá)的影響

   微小RNA（micro RNA，miRNA）是一類在轉(zhuǎn)錄后水平調(diào)節(jié)基因表達(dá)的非編碼小分子RNA，其調(diào)控基因表達(dá)、影響細(xì)胞活性的功能已為人們所重視。近年來miRNA的跨界調(diào)控成為新的研究方向，為人們更全面地認(rèn)識和了解miRNA的功能提供了新的視角。植物miRNA由于通常經(jīng)過了甲基化修飾，因此較為穩(wěn)定，不易降解。前期研究表明，甘草干燥藥材的水煎劑中存在著豐富的小分子RNA，這為了解甘草的作用機制提供了新的思路。在本研究中，利用從甘草水煎劑中提取的小分子RNA以及合成的miRNA模擬物（mimic）作用于分離自健康人的外周血單核細(xì)胞（peripheral blood mononuclear cell，PBMC），然后利用RT-PCR對其模式識別受體（PRR）基因以及部分轉(zhuǎn)錄因子、信號分子和細(xì)胞因子的基因表達(dá)變化進行了分析。結(jié)果表明甘草miRNA對人免疫細(xì)胞的基因表達(dá)具有明顯的調(diào)節(jié)作用，能夠顯著抑制T細(xì)胞分化相關(guān)基因的表達(dá)，以及炎癥和凋亡相關(guān)基因的表達(dá)。這為進一步更全面地了解甘草的作用機制以及中藥新藥的研制帶來了新的思路。

Effect of miRNA from Glycyrrhiza uralensis decoction on gene expression of human immune cells

   MicroRNAs(miRNA) are small non-coding RNAs that regulate the expression of protein coding genes by repressing translation of protein coding mRNA or enhancing mRNA degradation. Its functions have attracted more and more attention from the public. In recent years, the cross-border regulation of miRNA has become a new research direction, and provides a new perspective for people to comprehensively understand the functions of miRNA. Plant miRNA is usually methylated and not easy to degrade. According to our previous researches, there were abundant small RNAs in the decoction of dried liquorice, which provides a new way to study the mechanism of action of licorice. In this study, small RNAs extracted from Glycyrrhiza uralensis decoction and synthesized miRNA mimics were used to treat peripheral blood mononuclear cells (PBMC) isolated from healthy volunteers. The gene expression of toll-like receptors (TLRs), some transcription factors, signal molecules and cytokines were analyzed by RT-PCR. The results showed that glycyrrhiza miRNA could significantly regulate PBMC by inhibiting the expression of genes involved in T cell differentiation, inflammation and apoptosis. The study brought new ideas to us in comprehensively studying the mechanism of licorice and developing the traditional Chinese medicine.
 

 插播一條通知：上海勁馬全體員工已于2018年2月26日(農(nóng)歷正月十一)正式上班，并恢復(fù)一切業(yè)務(wù)！歡迎科研朋友們業(yè)務(wù)。在這里祝各位新老客戶新年快樂，狗年行大運，闔家歡樂，財源不斷，心想事成！