Description
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Analysis Note Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
  The NIH assay procedure uses 0.2 mL of diluted plasma （1:1 with saline） as a substrate and 0.1 mL of thrombin sample （stabilized in a 1% buffered albumin solution） based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Biochem/physiol Actions Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Other Notes View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Preparation Note Activated with bovine brain thromboplastin.
Physical form Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0

Properties
form lyophilized powder
composition Protein, 40-60% 
EQP level Premium
storage temp. −20°C

Safety
Safety Statements 22-24/25
WGK Germany 2
RTECS XO8950000

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