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Description
Other Notes Note: At the reported pH optimum of 8.8, we have found the activity to be approx. 10 times that at pH 7.0. However, to remain consistent with literature and avoid complications arising from non-enzymatic oxidation of GSH, our unit is defined at pH 7.0.
Unit Definition 1 U corresponds to the amount of enzyme which catalyzes the oxidation by H2O2 of 1 μmol reduced glutathione to oxidized glutathione per minute at pH 7.0 and 25°C
Properties
form powder
quality lyophilized
mol wt Mr ~80000
color white
shipped in dry ice
storage temp. −20°C
Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Safety Statements 22-24/25
WGK Germany 3
References
reference B. Mannervik Meth. Enzymol. 113, 490, (1985) Abstract
A. Meister, M.E. Anderson Annu. Rev. Biochem. 52, 711, (1983) Abstract
Tappel, A.L. Curr. Top. Cell Regul. 24, 87, (1984)
Mannervik, B. Meth. Enzymol. 113, 490, (1985)
Wendel, A., Jacoby, W.B., ed. Enzymatic Basis of Detoxification New York , (1980), 333
Flohe, L. Curr. Top. Cell Regul. 27, 473,, (1985)
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