Description
Application Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% （v/v） ethanol, 10% （v/v） acetic acid or in 7% acetic acid. The gel can be destained with either 30% （v/v） ethanol, 10% （v/v） acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Properties
composition Dye content, 90% 
mp 290 °C （dec.）（lit.）
UV absorption λmax 427 nm
  λmax 622 nm （2nd）

Safety
Personal Protective Equipment Eyeshields, Gloves
Hazard Codes Xn
Risk Statements 68
Safety Statements 36/37
WGK Germany 2
RTECS BQ4425000
 

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