Description
Application 3,3′,5,5′-Tetramethylbenzidine （TMB） is a noncarcinogenic substitute （Ames test negative） for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 （resulting in a yellow color）, and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8
Other Notes Standard for determining aromatic amines released from azo-dyes in commodities9

Properties
grade standard for GC
  puriss. p.a.
assay ≥99.0% （GC）
shelf life  （limited shelf life, expiry date on the label）
mp 166-171 °C
  168-171 °C（lit.）

Safety
Personal Protective Equipment dust mask type N95 （US）, Eyeshields, Faceshields, Gloves
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
WGK Germany 3
RTECS DV2300000

• S0178茚三酮/苯并戊三酮/水合茚三酮/苯駢戊三酮/苯并環(huán)丙三酮/2,2-二羥基-1H-茚并-1,3（2H）
• S0171氯化血紅素/血晶素/血紅素/卟啉鐵/Hematin chloride
• S01861-苯基-3-吡唑烷酮/菲尼酮/菲尼酮A/Phenidone
• S01851-苯基-3-甲基-5-吡唑酮/依達(dá)拉奉/3-甲基-1-苯基-2-吡唑啉-5-酮/3-甲基-1-苯基
• S01841-苯基-3-甲基-4-苯甲酰基吡唑啉酮
• S0183苯芴酮/鍺試劑/苯基芴酮/苯基熒光酮/2,6,7-三羥基-9-苯基熒光酮/2,6,7-三羥基-9-苯
• S0182米氏酮/4，4′-四甲基二氨基二苯甲酮/米蚩酮/米氣勒氏酮/四甲對(duì)，對(duì)′-二氨二苯甲酮/4,4′-雙
• S01814，4-雙（二甲氨基）硫代二苯甲酮/4,4-雙（二甲氨基）硫代二甲酮/硫代米氏酮/4,4′-四甲基二
• S0179蒽酮/9,10-二氫-9-氧蒽/Anthrone
• S01774-（4-硝基苯偶氮）-1-萘酚/4-（對(duì)硝基苯偶氮）-1-萘酚/對(duì)硝基偶氮苯-α-萘酚/鎂試劑II